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                                      Sperm Hypo-Osmotic Staining Kit

SPECIFICATION AND INTRODUCTION

[Description]

Generic name: Sperm Hypo-Osmotic Staining Kit

English name: Sperm Hypo-Osmotic Staining Kit

[Packing specification] each box for 20 tests

[Intended use] staining of vital sperm for evaluating sperm viability  

[Principle of test] when sperms are in hypo-osmotic solution, water molecules can pass through sperm membrane into the sperm and lead to increase volume and swelling of sperm. In particular, swelling of sperm’s tail is more obvious. Dead sperms or sperms with incomplete membrane usually don’t present swelling.

[Major components]

Solution A

[Storage condition and shelf life] -20℃; 12 months

[Applicable apparatus] optical microscope

[Sample required] fresh or frozen semen

[Test method]

(1)    Take a bottle of solution A and preheat it under 37℃ for 5 minutes;  

(2)     Add 0.1 ml of semen sample, blend for 30 minutes, shake well, drip the mixture into slide pool (approximately 4ul), and count the percentage of swelling sperms in 200 sperms.

[Reference value] HOS＜ 50 is considered as abnormal

[Explanation of test result] when sperms are in hypo-osmotic solution, water molecules can pass through sperm membrane into the sperm and lead to increase volume and swelling of sperm. In particular, swelling of sperm’s tail is more obvious. Dead sperms or sperms with incomplete membrane usually don’t present swelling.

[Limitation of test method] only applicable to detection of vital sperms

[Product performance indicator] when compared with similar foreign products, the product has a Spearman correlation coefficient of 0.654 ﹙P＜0.0001﹚.

[Precaution] Reagents of different batches cannot be mixed.

[References] WHO; Laboratory Manual for the Examination and Processing of Human Semen, the fifth edition

[Medical Device Manufacturing Enterprise License Number] No. 20120367

[Medical Device Registration Certificate Number] No. 1400218 (Approval) of 2013

[Product Standard Number] YZB/Hefei 0006-2013  

[Approval/revision date of the specification] 17 April 2013 
[Manufactured by]

Anhui Anke Biotechnology (Group) Co., Ltd

[Address]

Ankebio Buildings, 669, Changjiang Road, West ,Hefei, 230088, P.R. China.

Tel: +86-551-65319890, 65318811

Fax:+86-551-65319895

Website: m.fumian.net.cn

E-mail: info@ankebio.com